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NOTE: The Height from Apex parameter should equal the average penetration depth or the indentation in the force curve.

Enter the measured average Deformation into the Height 1 from Apex field.

Click the Tip Qualification icon in the NanoScope Analysis toolbar to open the Tip Qualification window.

Flatten the image by clicking the Plane Fit icon.

Open the Height channel of the saved image in the NanoScope Analysis package.

Because this is an intentionally rough sample that can damage the probe tip, set ScanAsyst Noise Threshold to 1.0 nm.

Characterizer image size is important because, along with Tip Image Size and feature density, it determines how many peaks are used for the tip estimation.

Set the Scan Size to approximately 1.5 mm. Scan ( Peak Force QNM in Air mode) the characterizer sample.Refer to the NanoScope Software User Guide for details. Tip radius may be measured using a tip characterizer sample and the Tip Qualification function in NanoScope or NanoScope Analysis software (NanoScope software does not include the Estimated End Radius function).

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